Wednesday, March 24

Opening Session

Moderator – Mark Pandori, PhD, San Francisco Department of Public Health, HIV Diagnostics Conference Planning Committee Chair

Evolution of HIV Diagnostics and Goals of the 2010 Conference

• Bernard Branson, MD, Centers for Disease Control and Prevention

HIV Testing in Public Health Laboratories: Results from the 2009 APHL HIV Testing Practices Survey

• Barbara Werner, PhD, Massachusetts Department of Health

Model Performance Evaluation Program (MPEP) HIV Rapid Testing Survey: Report of Sample Shipment Results, September 2009

• Steven Ethridge MT(ASCP), Centers for Disease Control and Prevention

 

Rapid Testing Algorithms at the Point of Care/Contact

Moderator – Kevin Delaney, MPH, Centers for Disease Control and Prevention

Lessons Learned from San Francisco’s Experience with Rapid Testing Algorithms

• Thomas Knoble, MSW, San Francisco Department of Public Health

Two-Rapid Test Strategy in Anonymous HIV Counseling and Testing (ACT) Sites in New York State 2008-2009

• April Richardson-Moore, RN, MPH, New York State Department of Health

Comparison of multiple laboratory-performed rapid HIV tests with standard confirmatory assays for assessment of multi-rapid test algorithms

• Linda Styer, PhD, Wadsworth Center, New York

Statewide Implementation of Rapid-Rapid Testing in New Jersey

• Eugene G. Martin, PhD, UMDNJ – Robert Wood Johnson Medical School

 

Rapid Testing Algorithms at the Point of Care/Contact

Moderator – Michael Pentella, PhD, D(ABMM), University of Iowa Hygienic Laboratory

Use of a three rapid HIV test algorithm at point-of-care settings to facilitate same day receipt of a final HIV test result, referral to medical care, and address false-positive screening tests

• Jacqueline Rurangirwa, MPH, County of Los Angeles, Department of Public Health, Office of AIDS Programs and Policy

Examination of an alternative algorithm for HIV diagnostics using HIV-1/2 rapid tests as confirmatory assays

• Silvina Masciotra, MS, Centers for Disease Control and Prevention

Expanded HIV Rapid Testing in Emergency Departments (ED) – Chicago, Illinois 2007-2008

• Nancy R. Glick, MD, Mt. Sinai Hospital, Chicago, IL
• Karen Reitan, MA, Public Health Institute of Metropolitan Chicago

Multiplexed POC Device for HIV and Co-Infection Serodiagnosis

• Michael Lochhead, PhD, mBio Diagnostics , Inc.

 

Bridging Point-of-Care/Contact & Laboratory Testing

Moderator – Kathleen Krchnavek, MSSW, Wisconsin Department of Health

Establishing Ongoing Competency Assessment to Maintain HIV Testing Skills

• Teri Dowling, MA, MPH, San Francisco Department of Public Health

A Successful HIV Testing Quality Assurance Program; New York State Experience

• Mara L. San Antonio-Gaddy, RN, MSN, New York State Department of Health

The Potential Effect of Alternative Algorithms on HIV Surveillance

• Kristen Mahle, MPH, Centers for Disease Control and Prevention

The HIV Bridge Algorithm: Linking Point-of-Care, Laboratory and Patient Care

• Berry Bennett, MPH, Florida Bureau of Laboratories

Thursday, March 25

Serologic Testing Methods

Moderator – William A. Meyer III, PhD, D(ABMM), Quest Diagnostics

Using all the data: Immunoassay signal-to-cutoff values provide useful information that should be considered in HIV diagnostic algorithms

• Kevin P. Delaney, MPH, Centers for Disease Control and Prevention

False-positive HIV Test Results Using a Peptide-based Enzyme Immunoassay (EIA) in Pregnant Women and Others

• Laura G. Wesolowski, PhD, Centers for Disease Control and Prevention

Dramatic Reduction in Indeterminate Results from HIV Antibody Testing of Blood Donors by Conversion from Western Blot to an Immunofluorescence Assay

• Michael P. Busch, MD, PhD, Blood Systems Research Institute and UCSF

Use of Multispot (MS) HIV-1/HIV-2 Rapid Test to confirm HIV-1/HIV-2 Plus O Enzyme immunoassay (EIA) results shorten reporting time for HIV testing and identify cryptic HIV-2 infection

• Robert W. Coombs, MD, PhD, FRCPC, University of Washington

 

Testing for Recent HIV Infection

Moderator – S. Michele Owen, PhD, Centers for Disease Control and Prevention

Recent advances in development and application of assays/algorithms for detection of recent HIV infections and estimation of incidence

• Michael P. Busch, MD, PhD, Blood Systems Research Institute and UCSF

Optimization and Calibration of Less Sensitive and Avidity Modified Protocols for the VITROS Immunodiagnostic Products Anti-HIV1+2 assay for Detection of Early HIV Infections and Incidence Estimation

• Sheila M. Keating, PhD, MSPH, Blood Systems Research Institute

Antibody avidity-based assay for identifying recent HIV-1 infections based on Genetic Systems TM 1/2 plus O EIA

• Silvina Masciotra, MS, Centers for Disease Control and Prevention

IgG3 as a Biomarker for Distinguishing Recent from Established HIV-1 Infection

• Kelly A. Curtis, PhD, Centers for Disease Control and Prevention

 

A Global Perspective: International Laboratory Poster Highlights

Moderators – Kenneth Landgraf, MS & Tony Tran, MPH, MT(ASCP), Association of Public Health Laboratories

Evaluation of Dried Blood Spots (DBS) for Human Immunodeficiency Virus (HIV-1) Drug Resistance Testing

• Dawit Assefa, Ethiopian Health & Nutrition Research Institute

HIV EQAS - Novel Perspectives

• Elizabeth Dax, National Serology Reference Laboratory, Australia and Indonesian Collaborators

SMARTube as a Test for Recent Infection

• Reshma Kassanjee, University of the Witwatersrand, South Africa

Comparison of Dried Tube and Plasma Specimens in an External Quality Assessment Scheme and Evaluation of Quality of Rapid Testing Performed by Non-Laboratory Staff in Lesotho

• Mathabo Lebina, Laboratory Services, Ministry of Health and Social Welfare, Lesotho

The High Positive Immunoassay Confirms HIV-1 Infection and Avoids the Need of Confirmatory Tests

• Rodolfo Ochoa-Jimenez, Regional Hospital of Colima, Mexico

Fourth Generation Immunoassays

Moderator – Bernard Branson, MD, Centers for Disease Control and Prevention

Performance of the AxSym Ag/Ab HIV Combo assay, a 4th generation assay for routine HIV screening

• Christiane Claessens, MSc, INSPQ/LSPQ, Sainte-Anne-de-Bellevue, QC, Canada

Performance Evaluation of the Bio-Rad GS HIV Combo Ag/Ab EIA

• Kathleen Shriver, PhD, Bio-Rad Laboratories

Development* of an HIV-1/2/O Antigen-Antibody Combo Assay for Use on Ortho Clinical Diagnostics VITROS® Integrated and Immunodiagnostic Systems

• Patrick Kilmartin, B.Sc., M.Sc., Ortho Clinical Diagnostics

Clinical and Analytical Evaluation of the ARCHITECT®HIV Ag/Ab Combo Assay

• Barbara A. Kaesdorf, MS, MT(ASCP), CCRA, Abbott Diagnostics

Assessing the sensitivities of laboratory-based and point-of-care HIV antigen-antibody combination tests using a panel of specimens from recently and acutely infected individuals

• Mark Pandori, PhD, San Francisco Department of Public Health

 

Applications of Nucleic Acid Testing Technologies

Moderator – Sally Liska, DrPH, San Francisco Department of Public Health

Optimization of Abbott m2000 RealTime HIV-1 Viral Load Assay on Breastmilk, Dried Blood Spots, Genital Secretions, Cerebrospinal Fluid, Urine, and Throatwash

• Julie A. E. Nelson, PhD, University of North Carolina at Chapel Hill

Evaluating Kit-Based HIV-1 DNA PCR Protocol for Confirming Infection

• Carolyn D. Dawson, BS, Centers for Disease Control and Prevention

New HIV-1 RNA Assay Validation: Roche COBAS TaqMan and Abbott RealTime

• James Bremer, PhD, Rush University

Point-of-Care Molecular Diagnostics

• Marco Schito, PhD, Henry Jackson Foundation, DAIDS, NIH, Bethesda, MD

Friday, March 26

New Approaches in HIV Testing

Moderator – Steven Ethridge, MT(ASCP), Centers for Disease Control and Prevention

ELISA Strength of Signal in a Protocol to Prevent Mother-to-Child HIV Transmission

• Michael Loeffelholz, PhD, ABMM, University of Texas Medical Branch

Rapid Detection of HIV-1 p24 Antigen and of Antibodies to HIV-1 and HIV-2 Using Magnetic Immunochromatography

• Timothy C. Granade, MS, Centers for Disease Control and Prevention

Europium Nanoparticle-based Immunoassay (ENIA) for Sensitive and Early Detection of Human Immunodeficiency Virus Type 1 Capsid (p24) Antigen

• Shixing Tang, MD, PhD, Food and Drug Administration

A novel method of creating molecular control used in the NLHRS HIV-2 Viral Load Assay and subsequent results in a NIBSC-WHO study to evaluate candidate international standards for HIV-2 RNA

• John Kim, PhD, National Laboratory for HIV Reference Services, Public Health Agency of Canada

 

Conference Wrap-Up & Next Steps

Moderated by Bernard Branson, MD, Centers for Disease Control and Prevention