Tuesday, December 11, 2012

HIV Testing Technologies
S. Michele Owen
Link to Presentation: S. Michele Owen

Specimens for HIV Testing
Richard L. Hodinka
Link to Presentation: Richard Hodinka

Evaluating HIV Test Performance

Bernard Branson
Link to Presentation: Bernard Branson

Why HIV Tests are Regulated as They Are, and Understanding the Package Insert
Elliot Cowan
Link to Presentation: Elliot Cowan

The Use of a Smart Phone Application to Disseminate Guidance on the Proposed Laboratory-based HIV Testing Algorithm
Link to Presentation: Nancy Cornish

Wednesday, December 12, 2012

Opening Session
Draft Algorithm Recommendations
Bernard Branson
Link to Presentation: Bernard Branson

Identification of Early HIV Infections Using the Fourth Generation Abbott Architect HIV Ag/Ab Combo Chemiluminescent Microparticle Immunoassay (CIA) in San Diego County
Anna Liza Manlutac and P. McVay
Link to Presentation: Anna Liza Manlutac
Link to Abstract: Review page 1

Performance Comparison of the 4th Generation Bio-Rad Laboratories GS HIV Combo Ag/Ab EIS on the Evolis Automated System versus Abbott Architect HIV Ag/Ab Combo, Ortho Anti HIV-1/HIV-2 EIA on Vitros ECi and Siemens HIV-1/O/2 Enhanced on ADVIA Centaur
Elizabeth Mitchell, G. Stewart, O. Bajzik, M. Ferret and M.K. Shriver
Link to Presentation: Elizabeth Mitchell
Link to Abstract: Review page 2

ARCHITECT® HIV Ag/Ab Combo Assay: Correlation of HIV-1 p24 Antigen Sensitivity and RNA Viral Load Using Genetically Diverse Virus Isolates
Catherine Brennan, J. Yamaguchi, A. Vallari, P. Swanson and J. Hackett
Link to Presentation: Catherine Brennan
Link to Abstract: Review page 8

4th Generation HIV Screening in Massachusetts: A Partnership Between Laboratory and Program
Tammy Goodhue, A. Kazianis, T. Stiles, M. Gaucher, B. Callis, H.D. Fukuda and K. Cranston
Link to Presentation: Tammy Goodhue
Link to Abstract: Review page 9

Session 2: 4th Generation HIV-1/HIV-2 Immunoassays Under Development

A Multicenter Performance Evaluation of the ADVIA Centaur HIV Ag/Ab Combo (CHIV) Assay on the ADVIA Centaur Immunoassay System
Lawrence Baker
Link to Presentation: Lawrence Baker
Link to Abstract: Review page 14

Performance of the VITROS ® Immunodiagnostic Products HIV Combo Assay
Charles Noeson
Link to Presentation: Charles Noeson
Link to Abstract: Review page 15

Performance of the Alere Determine™ HIV-1/2 Ag/Ab Combo Rapid Test with Specimens from U.S. HIV-1 Seroconverters and HIV-2 Positive Specimens from Ivory Coast
Silvina Masciotra, W. Luo, A. Youngpairoj, M. Kennedy and S.M. Owen
Link to Presentation: Silvina Masciotra
Link to Abstract: Review page 16

Session 3: HIV Testing Algorithms in the Laboratory (Part 1)

Performance of an HIV Diagnostic Algorithm Using the Architect HIV Ag/Ab Combo Assay and Potential Utility of Sample-to-Cutoff Ratio
Robert Coombs
Link to Presentation: Robert Coombs
Link to Abstract: Review page 17

Evaluation of the Implementation of the 4th Generation HIV Combo/Multispot Assay Algorithm in Alaska, A Low HIV Prevalence State
Catherine Humphries
Link to Presentation: Catherine Humphries
Link to Abstract: Review page 20


Performance of the New HIV-1/2 Diagnostic Algorithm in Florida’s Public Health Testing Population: A Review of the First Five Months of Utilization
Dana Neumann, B. Bennett and L. Gillis
Link to Presentation: Dana Neumann
Link to Abstract: Review page 22


Session 4: HIV Testing Algorithms in the Laboratory (Part 2)

Clinical Performance of the Multispot HIV-1/HIV-2 Rapid Test to Identify HIV-2 Infection and Cross Reactivity with HIV-1 Western Blot
Eric Ramos, S. Harb, J. Dragavon and R. Coombs
Link to Presentation: Eric Ramos
Link to Abstract: Review page 23

Evaluation of an Alternative Algorithm for HIV Diagnosis Among Men Who Have Sex with Men (MSM) in Five U.S. Cities – 2011
Silvina Masciotra, A. Smith, A. Youngpairoj, P. Sprinkle, I. Miles, C. Sionean, G. Paz-Bailey, J. Johnson and S.M. Owen
Link to Presentation: Silvina Masciotra
Link to Abstract: Review page 25

Performance of the U.S. Army HIV Diagnostics Algorithm
Sheila Peel, M. Manak, K. Rice, E. Quick, J. Malia and Y. Beale
Link to Presentation: Abstract is available in program. Slides are not available.
Link to Abstract: Review page 26

Evaluation of Supplemental Testing with the Multispot HIV-1/HIV-2 Rapid Test and APTIMA HIV-1 RNA Qualitative Assay to Resolve Specimens with Indeterminate or Negative HIV-1 Western Blots
Laurie Linley, S. Ethridge, E. Oraka, S.M. Owen, L. Wesolowski, K. Wroblewski, K. Landgraf, M. Parker, M. Brinson and B. Branson
Link to Presentation: Abstract is available in program. Slides are not available.
Link to Abstract: Review page 27

The Multispot Rapid HIV-1/HIV-2 Differentiation Assay is Comparable with the Western Blot and an Immunofluorescence Assay at Confirming HIV Infection in a Prospective Study in Three Regions of the United States
Mark W. Pandori, E. Westheimer, C. Gay, N. Moss, J. Fu, L. Hightow-Weidman, J. Craw, L. Hall, F. Giancotti, J. Embry, B. Louie, P. Patel, S.M. Owen and P. Peters
Link to Presentation: Mark Pandori
Link to Abstract: Review page 28

The Route to Implementation of the New HIV Diagnostic Algorithm in New York State
Monica Parker, B. Anderson, L. Smith, A. Muse, L. Styer and J. Wikoff
Link to Presentation: Monica Parker
Link to Abstract: Review page 30


Session 5: Testing for HIV/Hepatitis or HIV/Syphilis Co-infections

HIV/Hepatitis Co-infection Rate in an Anonymous Unlinked Seroprevalence Survey in an Urban Hospital
Debbie Mohammed, E. Martin, S. Paul, C. Sadashigie and S. Scott
Link to Presentation: Debbie Mohammed
Link to Abstract: Review page 45

Performance Evaluation of the DPP® HIV-SYPHILIS Assay: A Novel, Point-of-care Rapid HIV 1/2 and Syphilis Treponema pallidum Antibody
Combination Test
Javan Esfandiari, D. Gunasekera, F. Sayegh, K. Addiss, A. Sikar-Gang, C. Shi, L. Sigismondi and K. Keskinen
Link to Presentation: Javan Esfandiari
Link to Abstract: Review page 46

Thursday, December 13, 2012

Session 6: Alternatives for Diagnostic Decision Making

Accuracy of Anti-HIV 1+2 Signal-to-Cutoff Ratio in Predicting HIV-1 Confirmatory Test Results
Lixia Liu, J. Gentry and J. Lovchik
Link to Presentation: Lixia Liu
Link to Abstract: Review page 48

Utility of Multispot HIV-1 Spot Reaction Intensity and Immunoassay S/CO Ratio for Determining HIV Infection Status
Linda Styer, T. Sullivan and M. Parker
Link to Presentation: Linda Styer
Link to Abstract: Review page 49

Cost and Effects of the APHL/CDC Proposed Laboratory-based Algorithm for the Detection of HIV
Angela Hutchinson, S. Ethridge, L. Wesolowski, P. Farnham, R. Shrestha, P. Patel and B. Branson
Link to Presentation: Abstract is available in program. Slides are not available.
Link to Abstract: Review page 50 and 52
(Combined Presentation)

Session 7: Nucleic Acid for Diagnosis and Prognosis

HIV Single Stage Testing: Integration and Maximization of Resources by Reducing Time Between HIV Diagnosis and Treatment
Berry Bennett and S. Fordan
Link to Presentation: Berry Bennett
Link to Abstract: Review page 56

Unique Testing Experience for Acute HIV Infection: The Dallas County NAAT Program
Brian Emerson
Link to Presentation: Brian Emerson
Link to Abstract: Review page 57

Validation and Clinical Use of a HIV-2 Viral Load Assay
Linda Styer, T. Miller and M. Parker
Link to Presentation: Linda Styer
Link to Abstract: Review page 58

Detection of HIV-2 Proviral DNA by Using Single or Multiplex Real-time PCR
Tim Granade, C. Pau, S. Wells, S.M. Owen and A. Youngpairoj
Link to Presentation: Tim Granade
Link to Abstract: Review page 59

Development of Real-Time PCR Assays for Detection of Integrated and Total HIV-1 DNA
Sheila Peel, L. Jagodzinski, C.Kibirige, H. Hack, Y. Liu and M. Manak
Link to Presentation: Abstract is available in program. Slides are not available.
Link to Abstract: Review page 60

Session 8: HIV Testing Outside the Laboratory

Evaluation of the Accuracy and Ease of Use of a Rapid, 60 Second HIV Test Performed by Untrained Operators in POC Test Centers in the United States
Rick Galli, K. Green, A. La Marca, L. Waldman and R. Powers
Link to Presentation: Rick Galli
Link to Abstract: Review page 63

Expansion of HIV Screening to Non-clinical Venues is Aided by Use of Dried Blood Spots for Western Blot Confirmation
Timothy Sullivan, M. San Antonio-Gaddy, L. Styer, A. Richardson-Moore, D. Bigelow-Saulsbery and M. Parker
Link to Presentation: Timothy Sullivan
Link to Abstract: Review page 69

Rapid-2-Rapid – A Collaborative Approach to Linkage
Eugene Martin, S. Paul, G. Salaru, E. Cadoff, L. Berezny, L. Dutton, S. Saudners and J. Corbo
Link to Presentation: Eugene Martin
Link to Abstract: Review page 70

Session 9: Laboratory and Surveillance Issues

Challenges in the Reporting of HIV-1 and HIV-2 Laboratory Results in Surveillance
Kristen Mahle Gray
Link to Presentation: Kristen Mahle Gray
Link to Abstract: Review page 80

CDC Update: HIV-2 Surveillance
M. Patricia Joyce
Link to Presentation: M. Patricia Joyce

CDC Update: Stage 0 HIV Infection Case Definition
Richard Selik
Link to Presentation: Richard Selik

Session 10: Testing Oral Fluid Specimens

Comparative Performance of the Avioq HIV-1 Microelisa System on Oral Fluid Specimens
Berry Bennett, S. Fordan and D. Neumann
Link to Presentation: Berry Bennett
Link to Abstract: Review page 85

Comparison of HIV Oral Fluid and Plasma Testing During Early Infection in a Longitudinal Nigerian Cohort
Wei Luo, S. Masciotra, K. Delaney, M. Charurat, T. Croxton, N. Constantine, A. Nasidi, W. Blattner, L. Wesolowski and S.M. Owen
Link to Presentation: Wei Luo
Link to Abstract: Review page 86

Oral Fluid is Inferior to Fingerstick Point-of-care HIV Tests Among Seattle MSM
Joanne Stekler, A. Lane, J. O’Neal and M. Golden
Link to Presentation: Abstract is available in program. Slides are not available.
Link to Abstract: Review page 87

Friday, December 14, 2012

Session 11: Assays Under Development

DPP Supplemental Rapid Test Data can Discriminate Recent from Longstanding HIV Infection
Chris Pilcher, R. Kassanjee, L. Motta, S. Facente, S. Keating, E. McKinney, F. Hecht, R. Sperhacke, M. Busch, G. Murphy, J. Esfandiari and A. Welte
Link to Presentation:Abstract is available in program. Slides are not available.
Link to Abstract: Review page 88

Development of Global HIV Subtype Reference Panels for Molecular Testing
Michael Busch, T. Denny, F. Gao, A. Sanchez and I. Hewlett
Link to Presentation: Michael Busch
Link to Abstract: Review page 89

New HIV Peptide-based Immunoassay Resolves Vaccine Induced Seropositivity in HIV Vaccine RV144 and HVTN204 Trials
Oksana Penezina, D. Clapham, V. Kovalenko, I. Rodriguez-Chavez, M. Busch, K. Hennessy, D. Crane, N. Krueger and A. Levin
Link to Presentation: Oksana Penezina
Link to Abstract: Review page 90

Isothermal Amplification of Diverse HIV-1 Subtypes by RT-LAMP
Kelly Curtis, D. Rudolph and S.M. Owen
Link to Presentation: Kelly Curtis
Link to Abstract: Review page 91

Development of Rapid and Sensitive Point-of-Care HIV Antigen/Antibody Tests Using Microfluidic Europium Nanoparticle Immunoassays
Jikun Liu, B. Du, P. Zhang, M. Haleyurgirlsetty, J. Zhao, R. Viswanath, O. Wood, S. Lee and I. Hewlett
Link to Presentation: Abstract is available in program. Slides are not available.
Link to Abstract: Review page 92

Closing Session
2012 HIV Diagnostics Conference Research Findings Relevant for the New HIV Testing Algorithm
Bernard Branson
Link to Presentation: Bernard Branson